LSBio
TBC1D9B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TBC1D9B Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all TBC1D9B antibodies →
$725.00each
Catalog Number: LS-G113513-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TBC1D9B (NM_015043), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
TBC1 domain family member 9B | TBC1D9B | KIAA0676
Origin Species
Human
NCBI GenBank
BC000701
SwissProt
Q66K14

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
138.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MWLSPEEVLV ANALWVTERA NPFFVLQRRR GHGRGGGLTG LLVGTLDVVL DSSARVAPYR
61ILHQTQDSQV YWTVACGSSR KEITKHWEWL ENNLLQTLSI FDSEEDITTF VKGKIHGIIA
121EENKNLQPQG DEDPGKFKEA ELKMRKQFGM PEGEKLVNYY SCSYWKGRVP RQGWLYLTVN
181HLCFYSFLLG KEVSLVVQWV DITRLEKNAT LLFPESIRVD TRDQELFFSM FLNIGETFKL
241MEQLANLAMR QLLDSEGFLE DKALPRPIRP HRNISALKRD LDARAKNECY RATFRLPRDE
301RLDGHTSCTL WTPFNKLHIP GQMFISNNYI CFASKEEDAC HLIIPLREVT IVEKADSSSV
361LPSPLSISTK SKMTFLFANL KDRDFLVQRI SDFLQKTPSK QPGSIGSRKA SVVDPSTESS
421PAPQEGSEQP ASPASPLSSR QSFCAQEAPT ASQGLLKLFQ KNSPMEDLGA KGAKEKMKEE
481SWHIHFFEYG RGVCMYRTAK TRALVLKGIP ESLRGELWLL FSGAWNEMVT HPGYYAELVE
541KSTGKYSLAT EEIERDLHRS MPEHPAFQNE LGIAALRRVL TAYAFRNPTI GYCQAMNIVT
601SVLLLYGSEE EAFWLLVALC ERMLPDYYNT RVVGALVDQG IFEELTRDFL PQLSEKMQDL
661GVISSISLSW FLTLFLSVMP FESAVVIVDC FFYEGIKVIL QVALAVLDAN MEQLLGCSDE
721GEAMTMLGRY LDNVVNKQSV SPPIPHLRAL LSSSDDPPAE VDIFELLKVS YEKFSSLRAE
781DIEQMRFKQR LKVIQSLEDT AKRSVVRAIP VDIGFSIEEL EDLYMVFKAK HLASQYWGCS
841RTMAGRRDPS LPYLEQYRID ASQFRELFAS LTPWACGSHT PLLAGRMFRL LDENKDSLIN
901FKEFVTGMSG MYHGDLTEKL KVLYKLHLPP ALSPEEAESA LEAAHYFTED SSSEEALPQE
961EQEGSGSEER GEEKGTSSPD YRHYLRMWAK EKEAQKETIK DLPKMNQEQF IELCKTLYNM
1021FSEDPMEQDL YHAIATVASL LLRIGEVGKK FSARTGRKPR DCATEEDEPP APELHQDAAR
1081ELQPPAAGDP QAKAGGDTHL GTAPQESQVV VEGGSGEGQG SPSQLLSDDE TKDDMSMSSY
1141SVVSTGSLQC EDLADDTVLV GGEACSPTAR IGGTVDTDWC ISFEQILASI LTESVLVNFF
1201EKRVDIGLKI KDQKKVERQF STASDHEQPG VSG
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

1233 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.