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TBC1D9 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TBC1D9 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all TBC1D9 antibodies →
$504.00each
Catalog Number: LS-G113434-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TBC1D9 (NM_015130), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA0882 | MDR1 | TBC1 domain family member 9 | TBC1 domain family member 9A | TBC1D9 | TBC1 domain family | member 9 | TBC1D9A
Origin Species
Human
NCBI GenBank
AB020689
SwissProt
Q6ZT07

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
143.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MWVNPEEVLL ANALWITERA NPYFILQRRK GHAGDGGGGG GLAGLLVGTL DVVLDSSARV
61APYRILYQTP DSLVYWTIAC GGSRKEITEH WEWLEQNLLQ TLSIFENEND ITTFVRGKIQ
121GIIAEYNKIN DVKEDDDTEK FKEAIVKFHR LFGMPEEEKL VNYYSCSYWK GKVPRQGWMY
181LSINHLCFYS FLMGREAKLV IRWVDITQLE KNATLLLPDV IKVSTRSSEH FFSVFLNINE
241TFKLMEQLAN IAMRQLLDNE GFEQDRSLPK LKRKSPKKVS ALKRDLDARA KSERYRALFR
301LPKDEKLDGH TDCTLWTPFN KMHILGQMFV STNYICFTSK EENLCSLIIP LREVTIVEKA
361DSSSVLPSPL SISTRNRMTF LFANLKDRDF LVQRISDFLQ QTTSKIYSDK EFAGSYNSSD
421DEVYSRPSSL VSSSPQRSTS SDADGERQFN LNGNSVPTAT QTLMTMYRRR SPEEFNPKLA
481KEFLKEQAWK IHFAEYGQGI CMYRTEKTRE LVLKGIPESM RGELWLLLSG AINEKATHPG
541YYEDLVEKSM GKYNLATEEI ERDLHRSLPE HPAFQNEMGI AALRRVLTAY AFRNPNIGYC
601QAMNIVTSVL LLYAKEEEAF WLLVALCERM LPDYYNTRVV GALVDQGVFE ELARDYVPQL
661YDCMQDLGVI STISLSWFLT LFLSVMPFES AVVVVDCFFY EGIKVIFQLA LAVLDANVDK
721LLNCKDDGEA MTVLGRYLDS VTNKDSTLPP IPHLHSLLSD DVEPYPEVDI FRLIRTSYEK
781FGTIRADLIE QMRFKQRLKV IQTLEDTTKR NVVRTIVTET SFTIDELEEL YALFKAEHLT
841SCYWGGSSNA LDRHDPSLPY LEQYRIDFEQ FKGMFALLFP WACGTHSDVL ASRLFQLLDE
901NGDSLINFRE FVSGLSAACH GDLTEKLKLL YKMHVLPEPS SDQDEPDSAF EATQYFFEDI
961TPECTHVVGL DSRSKQGADD GFVTVSLKPD KGKRANSQEN RNYLRLWTPE NKSKSKNAKD
1021LPKLNQGQFI ELCKTMYNMF SEDPNEQELY HATAAVTSLL LEIGEVGKLF VAQPAKEGGS
1081GGSGPSCHQG IPGVLFPKKG PGQPYVVESV EPLPASLAPD SEEHSLGGQM EDIKLEDSSP
1141RDNGACSSML ISDDDTKDDS SMSSYSVLSA GSHEEDKLHC EDIGEDTVLV RSGQGTAALP
1201RSTSLDRDWA ITFEQFLASL LTEPALVKYF DKPVCMMARI TSAKNIRMMG KPLTSASDYE
1261ISAMSG
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1266 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.