LSBio
TBC1D2B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TBC1D2B Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all TBC1D2B antibodies →
$725.00each
Catalog Number: LS-G127365-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TBC1D2B (NM_144572), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA1055 | TBC1 domain family | member 2B | TBC1D2B | TBC1 domain family member 2B
Origin Species
Human
NCBI GenBank
AB028978
SwissProt
Q9UPU7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
109.7 kda
Protein Type
Lysate
Amino Acid Sequence
1MPGAGARAEE GGGGGEGAAQ GAAAEPGAGP AREPARLCGY LQKLSGKGPL RGYRSRWFVF
61DARRCYLYYF KSPQDALPLG HLDIADACFS YQGPDEAAEP GTEPPAHFQV HSAGAVTVLK
121APNRQLMTYW LQELQQKRWE YCNSLDMVKW DSRTSPTPGD FPKGLVARDN TDLIYPHPNA
181SAEKARNVLA VETVPGELVG EQAANQPAPG HPNSINFYSL KQWGNELKNS MSSFRPGRGH
241NDSRRTVFYT NEEWELLDPT PKDLEESIVQ EEKKKLTPEG NKGVTGSGFP FDFGRNPYKG
301KRPLKDIIGS YKNRHSSGDP SSEGTSGSGS VSIRKPASEM QLQVQSQQEE LEQLKKDLSS
361QKELVRLLQQ TVRSSQYDKY FTSSRLCEGV PKDTLELLHQ KDDQILGLTS QLERFSLEKE
421SLQQEVRTLK SKVGELNEQL GMLMETIQAK DEVIIKLSEG EGNGPPPTVA PSSPSVVPVA
481RDQLELDRLK DNLQGYKTQN KFLNKEILEL SALRRNAERR ERDLMAKYSS LEAKLCQIES
541KYLILLQEMK TPVCSEDQGP TREVIAQLLE DALQVESQEQ PEQAFVKPHL VSEYDIYGFR
601TVPEDDEEEK LVAKVRALDL KTLYLTENQE VSTGVKWENY FASTVNREMM CSPELKNLIR
661AGIPHEHRSK VWKWCVDRHT RKFKDNTEPG HFQTLLQKAL EKQNPASKQI ELDLLRTLPN
721NKHYSCPTSE GIQKLRNVLL AFSWRNPDIG YCQGLNRLVA VALLYLEQED AFWCLVTIVE
781VFMPRDYYTK TLLGSQVDQR VFRDLMSEKL PRLHGHFEQY KVDYTLITFN WFLVVFVDSV
841VSDILFKIWD SFLYEGPKVI FRFALALFKY KEEEILKLQD SMSIFKYLRY FTRTILDARK
901LISISFGDLN PFPLRQIRNR RAYHLEKVRL ELTELEAIRE DFLRERDTSP DKGELVSDEE
961EDT
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

963 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.