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TBC1D25 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TBC1D25 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all TBC1D25 antibodies →
$725.00each
Catalog Number: LS-G117902-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TBC1D25 (NM_002536), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
5SN3 snoRNA | TBC1 domain family member 25 | MG81 | OATL1 | TBC1 domain family | member 25 | TBC1D25
Origin Species
Human
NCBI GenBank
L08240
SwissProt
Q3MII6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
76.1 kda
Protein Type
Lysate
Amino Acid Sequence
1MATASGASDL SGSGAPPPGV GAQAAAAAEE EEREVVRVRV KKCESFLPPE FRSFAVDPQI
61TSLDVLQHIL IRAFDLSGKK NFGISYLGRD RLGQEVYLSL LSDWDLSTAF ATASKPYLQL
121RVDIRPSEDS PLLEDWDIIS PKDVIGSDVL LAEKRSSLTT AALPFTQSIL TQVGRTLSKV
181QQVLSWSYGE DVKPFKPPLS DAEFHTYLNH EGQLSRPEEL RLRIYHGGVE PSLRKVVWRY
241LLNVYPDGLT GRERMDYMKR KSREYEQLKS EWAQRANPED LEFIRSTVLK DVLRTDRAHP
301YYAGPEDGPH LRALHDLLTT YAVTHPQVSY CQGMSDLASP ILAVMDHEGH AFVCFCGIMK
361RLAANFHPDG RAMATKFAHL KLLLRHADPD FYQYLQEAGA DDLFFCYRWL LLELKREFAF
421DDALRMLEVT WSSLPPDPPE HEVELVGPPS QVADAGFGGH RGWPVRQRHM LRPAGGGGST
481FEDAVDHLAT ASQGPGGGGR LLRQASLDGL QQLRDNMGSR RDPLVQLPHP AALISSKSLS
541EPLLNSPDPL LSSFSHPDSP SSSSPPSTQE ASPTGDMAVG SPLMQEVGSP KDPGKSLPPV
601PPMGLPPPQE FGRGNPFMLF LCLAILLEHR DHIMRNGLDY NELAMHFDRL VRKHHLGRVL
661RRARALFADY LQSEVWDSEE GAEATAAS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

688 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.