LSBio
TBC1D22B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TBC1D22B Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all TBC1D22B antibodies →
$725.00each
Catalog Number: LS-G112221-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TBC1D22B (NM_017772), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C6orf197 | DJ744I24.2 | RP4-744I24.2 | TBC1 domain family member 22B | TBC1 domain family | member 22B | TBC1D22B
Origin Species
Human
NCBI GenBank
NM_017772
SwissProt
Q9NU19

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
58.9 kda
Protein Type
Lysate
Amino Acid Sequence
1MAAENSKQFW KRSAKLPGSI QPVYGAQHPP LDPRLTKNFI KERSKVNTVP LKNKKASSFH
61EFARNTSDAW DIGDDEEEDF SSPSFQTLNS KVALATAAQV LENHSKLRVK PERSQSTTSD
121VPANYKVIKS SSDAQLSRNS SDTCLRNPLH KQQSLPLRPI IPLVARISDQ NASGAPPMTV
181REKTRLEKFR QLLSSQNTDL DELRKCSWPG VPREVRPITW RLLSGYLPAN TERRKLTLQR
241KREEYFGFIE QYYDSRNEEH HQDTYRQIHI DIPRTNPLIP LFQQPLVQEI FERILFIWAI
301RHPASGYVQG INDLVTPFFV VFLSEYVEED VENFDVTNLS QDMLRSIEAD SFWCMSKLLD
361GIQDNYTFAQ PGIQKKVKAL EELVSRIDEQ VHNHFRRYEV EYLQFAFRWM NNLLMRELPL
421RCTIRLWDTY QSEPEGFSHF HLYVCAAFLI KWRKEILDEE DFQGLLMLLQ NLPTIHWGNE
481EIGLLLAEAY RLKYMFADAP NHYRR
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

505 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.