LSBio
TBC1D22A Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TBC1D22A Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all TBC1D22A antibodies →
$504.00each
Catalog Number: LS-G114013-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TBC1D22A (NM_014346), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C22orf4 | HSC79E021 | Putative GTPase activator | TBC1 domain family | member 22A | TBC1D22A | TBC1 domain family member 22A
Origin Species
Human
NCBI GenBank
NM_014346
SwissProt
Q8WUA7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
59.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MASDGARKQF WKRSNSKLPG SIQHVYGAQH PPFDPLLHGT LLRSTAKMPT TPVKAKRVST
61FQEFESNTSD AWDAGEDDDE LLAMAAESLN SEVVMETANR VLRNHSQRQG RPTLQEGPGL
121QQKPRPEAEP PSPPSGDLRL VKSVSESHTS CPAESASDAA PLQRSQSLPH SATVTLGGTS
181DPSTLSSSAL SEREASRLDK FKQLLAGPNT DLEELRRLSW SGIPKPVRPM TWKLLSGYLP
241ANVDRRPATL QRKQKEYFAF IEHYYDSRND EVHQDTYRQI HIDIPRMSPE ALILQPKVTE
301IFERILFIWA IRHPASGYVQ GINDLVTPFF VVFICEYIEA EEVDTVDVSG VPAEVLCNIE
361ADTYWCMSKL LDGIQDNYTF AQPGIQMKVK MLEELVSRID EQVHRHLDQH EVRYLQFAFR
421WMNNLLMREV PLRCTIRLWD TYQSEPDGFS HFHLYVCAAF LVRWRKEILE EKDFQELLLF
481LQNLPTAHWD DEDISLLLAE AYRLKFAFAD APNHYKK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

517 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.