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TBC1D2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TBC1D2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all TBC1D2 antibodies →
$725.00each
Catalog Number: LS-G111738-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TBC1D2 (NM_018421), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Armus | PARIS-1 | PARIS1 | TBC1 domain family member 2A | TBC1D2 | PP8997 | TBC1 domain family | member 2 | member 2A | TBC1D2A
Origin Species
Human
NCBI GenBank
NM_018421
SwissProt
Q9BYX2

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
103.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEGAGENAPE SSSSAPGSEE SARDPQVPPP EEESGDCARS LEAVPKKLCG YLSKFGGKGP
61IRGWKSRWFF YDERKCQLYY SRTAQDANPL DSIDLSSAVF DCKADAEEGI FEIKTPSRVI
121TLKAATKQAM LYWLQQLQMK RWEFHNSPPA PPATPDAALA GNGPVLHLEL GQEEAELEEF
181LCPVKTPPGL VGVAAALQPF PALQNISLKH LGTEIQNTMH NIRGNKQAQG TGHEPPGEDS
241PQSGEPQREE QPLASDASTP GREPEDSPKP APKPSLTISF AQKAKRQNNT FPFFSEGITR
301NRTAQEKVAA LEQQVLMLTK ELKSQKELVK ILHKALEAAQ QEKRASSAYL AAAEDKDRLE
361LVRHKVRQIA ELGRRVEALE QERESLAHTA SLREQQVQEL QQHVQLLMDK NHAKQQVICK
421LSEKVTQDFT HPPDQSPLRP DAANRDFLSQ QGKIEHLKDD MEAYRTQNCF LNSEIHQVTK
481IWRKVAEKEK ALLTKCAYLQ ARNCQVESKY LAGLRRLQEA LGDEASECSE LLRQLVQEAL
541QWEAGEASSD SIELSPISKY DEYGFLTVPD YEVEDLKLLA KIQALESRSH HLLGLEAVDR
601PLRERWAALG DLVPSAELKQ LLRAGVPREH RPRVWRWLVH LRVQHLHTPG CYQELLSRGQ
661AREHPAARQI ELDLNRTFPN NKHFTCPTSS FPDKLRRVLL AFSWQNPTIG YCQGLNRLAA
721IALLVLEEEE SAFWCLVAIV ETIMPADYYC NTLTASQVDQ RVLQDLLSEK LPRLMAHLGQ
781HHVDLSLVTF NWFLVVFADS LISNILLRVW DAFLYEGTKY NEKEILRLQN GLEIYQYLRF
841FTKTISNSRK LMNIAFNDMN PFRMKQLRQL RMVHRERLEA ELRELEQLKA EYLERRASRR
901RAVSEGCASE DEVEGEA
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

917 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.