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TBC1D17 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TBC1D17 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all TBC1D17 antibodies →
$504.00each
Catalog Number: LS-G130086-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TBC1D17 (NM_001168222), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
TBC1D17 | TBC1 domain family member 17 | TBC1 domain family | member 17
Origin Species
Human
NCBI GenBank
NM_024682

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
69.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEGAGYRDND VLLHWAPVEE AGDSTQILFS KKDSSGGDSC ASEEEPTFDP GYEPDWAVIS
61TVRPQLCHSE PTRGAEPSCP QGSWAFSVSL GELKSIRRSK PGLSWAYLVL VTQAGGSLPA
121LHFHRGGTRA LLRVLSRYLL LASSPQDSRL YLVFPHDSSA LSNSFHHLQL FDQDSSNVVS
181RFLQDPYSTT FSSFSRVTNF FRGALQPQPE GAASDLPPPP DDEPEPGFEV ISCVELGPRP
241TVERGPPVTE EEWARHVGPE GRLQQVPELK NRIFSGGLSP SLRREAWKFL LGYLSWEGTA
301EEHKAHIRKK TDEYFRMKLQ WKSVSPEQER RNSLLHGYRS LIERDVSRTD RTNKFYEGPE
361NPGLGLLNDI LLTYCMYHFD LGYVQGMSDL LSPILYVIQN EVDAFWCFCG FMELVQGNFE
421ESQETMKRQL GRLLLLLRVL DPLLCDFLDS QDSGSLCFCF RWLLIWFKRE FPFPDVLRLW
481EVLWTGLPGP NLHLLVACAI LDMERDTLML SGFGSNEILK HINELTMKLS VEDVLTRAEA
541LHRQLTACPE LPHNVQEILG LAPPAEPHSP SPTASPLPLS PTRAPPTPPP STDTAPQPDS
601SLEILPEEED EGADS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

615 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.