LSBio
TBC1D16 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TBC1D16 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all TBC1D16 antibodies →
$504.00each
Catalog Number: LS-G111494-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TBC1D16 (NM_019020), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
TBC1 domain family | member 16 | TBC1 domain family member 16 | TBC1D16
Origin Species
Human
NCBI GenBank
NM_019020
SwissProt
Q8TBP0

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
86.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSLGRLLRRA SSKASDLLTL TPGGSGSGSP SVLDGEIIYS KNNVCVHPPE GLQGLGEHHP
61GYLCLYMEKD EMLGATLILA WVPNSRIQRQ DEEALRYITP ESSPVRKAPR PRGRRTRSSG
121ASHQPSPTEL RPTLTPKDED ILVVAQSVPD RMLASPAPED EEKLAQGLGV DGAQPASQPA
181CSPSGILSTV SPQDVTEEGR EPRPEAGEED GSLELSAEGV SRDSSFDSDS DTFSSPFCLS
241PISAALAESR GSVFLESDSS PPSSSDAGLR FPDSNGLLQT PRWDEPQRVC ALEQICGVFR
301VDLGHMRSLR LFFSDEACTS GQLVVASRES QYKVFHFHHG GLDKLSDVFQ QWKYCTEMQL
361KDQQVAPDKT CMQFSIRRPK LPSSETHPEE SMYKRLGVSA WLNHLNELGQ VEEEYKLRKA
421IFFGGIDVSI RGEVWPFLLR YYSHESTSEE REALRLQKRK EYSEIQQKRL SMTPEEHRAF
481WRNVQFTVDK DVVRTDRNNQ FFRGEDNPNV ESMRRILLNY AVYNPAVGYS QGMSDLVAPI
541LAEVLDESDT FWCFVGLMQN TIFVSSPRDE DMEKQLLYLR ELLRLTHVRF YQHLVSLGED
601GLQMLFCHRW LLLCFKREFP EAEALRIWEA CWAHYQTDYF HLFICVAIVA IYGDDVIEQQ
661LATDQMLLHF GNLAMHMNGE LVLRKARSLL YQFRLLPRIP CSLHDLCKLC GSGMWDSGSM
721PAVECTGHHP GSESCPYGGT VEMPSPKSLR EGKKGPKTPQ DGFGFRR
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

767 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.