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TBC1D15 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TBC1D15 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all TBC1D15 antibodies →
$504.00each
Catalog Number: LS-G110249-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TBC1D15 (NM_022771), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
GAP for RAB7 | RAB7-GAP | TBC1D15 | GTPase-activating protein RAB7 | TBC1 domain family member 15 | TBC1 domain family | member 15
Origin Species
Human
NCBI GenBank
AL157464

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
82 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAAAGVVSGK IIYEQEGVYI HSSCGKTNDQ DGLISGILRV LEKDAEVIVD WRPLDDALDS
61SSILYARKDS SSVVEWTQAP KERGHRGSEH LNSYEAEWDM VNTVSFKRKP HTNGDAPSHR
121NGKSKWSFLF SLTDLKSIKQ NKEGMGWSYL VFCLKDDVVL PALHFHQGDS KLLIESLEKY
181VVLCESPQDK RTLLVNCQNK SLSQSFENLL DEPAYGLIQA GLLDRRKLLW AIHHWKKIKK
241DPYTATMIGF SKVTNYIFDS LRGSDPSTHQ RPPSEMADFL SDAIPGLKIN QQEEPGFEVI
301TRIDLGERPV VQRREPVSLE EWTKNIDSEG RILNVDNMKQ MIFRGGLSHA LRKQAWKFLL
361GYFPWDSTKE ERTQLQKQKT DEYFRMKLQW KSISQEQEKR NSRLRDYRSL IEKDVNRTDR
421TNKFYEGQDN PGLILLHDIL MTYCMYDFDL GYVQGMSDLL SPLLYVMENE VDAFWCFASY
481MDQMHQNFEE QMQGMKTQLI QLSTLLRLLD SGFCSYLESQ DSGYLYFCFR WLLIRFKREF
541SFLDILRLWE VMWTELPCTN FHLLLCCAIL ESEKQQIMEK HYGFNEILKH INELSMKIDV
601EDILCKAEAI SLQMVKCKEL PQAVCEILGL QGSEVTTPDS DVGEDENVVM TPCPTSAFQS
661NALPTLSASG ARNDSPTQIP VSSDVCRLTP A
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

691 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.