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TBC1D12 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TBC1D12 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all TBC1D12 antibodies →
$725.00each
Catalog Number: LS-G113355-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TBC1D12 (NM_015188), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA0608 | TBC1 domain family member 12 | TBC1 domain family | member 12 | TBC1D12
Origin Species
Human
NCBI GenBank
AB011180
SwissProt
O60347

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
85.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MVGPEDAGAC SGRNPKLLPV PAPDPVGQDR KVIRATGGFG GGVGAVEPPE EADEEEEADE
61EEETPPRQLL QRYLAAAGEQ LEPGLCYCPL PAGQAGAPPP SAAPRSDACL LGSGSKHRGA
121EVADGRAPRH EGMTNGDSGF LPGRDCRDLE EARGLARAGG RESRRRRPYG RLRLEGPGDE
181DADGAGSPSD WASPLEDPLR SCCLVAADAQ EPEGAGSDSG DSPASSCSSS EDSEQRGVGA
241GGPEEGAPPA TSAERTNGGA EPRLGFSDIH FNSRNTFQVS RGQSARDHLP PAGPPVPLPA
301AEQGPAGASA RARRSGGFAD FFTRNLFPKR TKELKSVVHS APGWKLFGKV PPRENLQKTS
361KIIQQEYEAR TGRTCKPPPQ SSRRKNFEFE PLSTTALILE DRPSNLPAKS VEEALRHRQE
421YDEMVAEAKK REIKEAHKRK RIMKERFKQE ENIASAMVIW INEILPNWEV MRSTRRVREL
481WWQGLPPSVR GKVWSLAVGN ELNITPELYE IFLSRAKERW KSFSETSSEN DTEGVSVADR
541EASLELIKLD ISRTFPSLYI FQKGGPYHDV LHSILGAYTC YRPDVGYVQG MSFIAAVLIL
601NLEEADAFIA FANLLNKPCQ LAFFRVDHSM MLKYFATFEV FFEENLSKLF LHFKSYSLTP
661DIYLIDWIFT LYSKSLPLDL ACRVWDVFCR DGEEFLFRTG LGILRLYEDI LLQMDFIHIA
721QFLTKLPEDI TSEKLFSCIA AIQMQNSTKK WTQVFASVMK DIKEGDKNSS PALKS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

775 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.