Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TBC1D10C / CARABIN Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G103670-100
Catalog Number: LS-G103670
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human carabin(TBC1D10C) (NM_198517), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Carabin | EPI64C | TBC1 domain family member 10C | TBC1 domain family | member 10C | TBC1D10C
- Origin Species
- Human
- SwissProt
- Q8IV04
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 49.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAQALGEDLV QPPELQDDSS SLGSDSELSG PGPYRQADRY GFIGGSSAEP GPGHPPADLI61RQREMKWVEM TSHWEKTMSR RYKKVKMQCR KGIPSALRAR CWPLLCGAHV CQKNSPGTYQ121ELAEAPGDPQ WMETIGRDLH RQFPLHEMFV SPQGHGQQGL LQVLKAYTLY RPEQGYCQAQ181GPVAAVLLMH LPPEEAFWCL VQICEVYLPG YYGPHMEAVR LDAEVFMALL RRLLPHVHKH241LQQVGVGPLL YLPEWFLCLF ARSLPFPTVL RVWDAFLSEG ARVLFRVGLT LVRLALGTAE301QRGACPGLLE TLGALRAIPP AQLQEEAFMS QVHSVVLSER DLQREIKAQL AQLPDSAPGP361PPRPQVRLAG AQAIFEAQQL AGVRRGAKPE VPRIVVQPPE EPRPPRRKPQ TRGKTFHGLL421TRARGPPIEG PPRPQRGSTS FLDTRFTRTRPLEQKLISEEDLAANDILDYKDDDDKV
446 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.