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TBC1D10A Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TBC1D10A Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G109114-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TBC1D10A (NM_031937), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
AC004997.C22.2 | DJ130H16.1 | EBP50-PDZ interactor of 64 kD | DJ130H16.2 | EPI64 protein | EBP50-PDX interactor of 64 kDa | EPI64 | TBC1D10A | TBC1 domain family member 10A | TBC1 domain family | member 10A | Rab27A-GAP-alpha | member 10 | TBC1D10
Origin Species
Human
NCBI GenBank
NM_031937
SwissProt
Q9BXI6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
56.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAKSNGENGP RAPAAGESLS GTRESLAQGP DAATTDELSS LGSDSEANGF AERRIDKFGF
61IVGSQGAEGA LEEVPLEVLR QRESKWLDML NNWDKWMAKK HKKIRLRCQK GIPPSLRGRA
121WQYLSGGKVK LQQNPGKFDE LDMSPGDPKW LDVIERDLHR QFPFHEMFVS RGGHGQQDLF
181RVLKAYTLYR PEEGYCQAQA PIAAVLLMHM PAEQAFWCLV QICEKYLPGY YSEKLEAIQL
241DGEILFSLLQ KVSPVAHKHL SRQKIDPLLY MTEWFMCAFS RTLPWSSVLR VWDMFFCEGV
301KIIFRVGLVL LKHALGSPEK VKACQGQYET IERLRSLSPK IMQEAFLVQE VVELPVTERQ
361IEREHLIQLR RWQETRGELQ CRSPPRLHGA KAILDAEPGP RPALQPSPSI RLPLDAPLPG
421SKAKPKPPKQ AQKEQRKQMK GRGQLEKPPA PNQAMVVAAA GDACPPQHVP PKDSAPKDSA
481PQDLAPQVSA HHRSQESLTS QESEDTYL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

508 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.