Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TBC1 / TBC1D1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G113432-100
Catalog Number: LS-G113432
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TBC1D1 (NM_015173), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- TBC1D1 | TBC | TBC1 | TBC1 domain family member 1 | KIAA1108
- Origin Species
- Human
- NCBI GenBank
- AB029031
- SwissProt
- Q86TI0
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 132.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MEPITFTARK HLLSNEVSVD FGLQLVGSLP VHSLTTMPML PWVVAEVRRL SRQSTRKEPV61TKQVRLCVSP SGLRCEPEPG RSQQWDPLIY SSIFECKPQR VHKLIHNSHD PSYFACLIKE121DAVHRQSICY VFKADDQTKV PEIISSIRQA GKIARQEELH CPSEFDDTFS KKFEVLFCGR181VTVAHKKAPP ALIDECIEKF NHVSGSRGSE SPRPNPPHAA PTGSQEPVRR PMRKSFSQPG241LRSLAFRKEL QDGGLRSSGF FSSFEESDIE NHLISGHNIV QPTDIEENRT MLFTIGQSEV301YLISPDTKKI ALEKNFKEIS FCSQGIRHVD HFGFICRESS GGGGFHFVCY VFQCTNEALV361DEIMMTLKQA FTVAAVQQTA KAPAQLCEGC PLQSLHKLCE RIEGMNSSKT KLELQKHLTT421LTNQEQATIF EEVQKLRPRN EQRENELIIS FLRCLYEEKQ KEHIHIGEMK QTSQMAAENI481GSELPPSATR FRLDMLKNKA KRSLTESLES ILSRGNKARG LQEHSISVDL DSSLSSTLSN541TSKEPSVCEK EALPISESSF KLLGSSEDLS SDSESHLPEE PAPLSPQQAF RRRANTLSHF601PIECQEPPQP ARGSPGVSQR KLMRYHSVST ETPHERKDFE SKANHLGDSG GTPVKTRRHS661WRQQIFLRVA TPQKACDSSS RYEDYSELGE LPPRSPLEPV CEDGPFGPPP EEKKRTSREL721RELWQKAILK QILLLRMEKE NQKLQASEND LLNKRLKLDY EEITPCLKEV TTVWEKMLST781PGRSKIKFDM EKMHSAVGQG VPRHHRGEIW KFLAEQFHLK HQFPSKQQPK DVPYKELLKQ841LTSQQHAILI DLGRTFPTHP YFSAQLGAGQ LSLYNILKAY SLLDQEVGYC QGLSFVAGIL901LLHMSEEEAF KMLKFLMFDM GLRKQYRPDM IILQIQMYQL SRLLHDYHRD LYNHLEEHEI961GPSLYAAPWF LTMFASQFPL GFVARVFDMI FLQGTEVIFK VALSLLGSHK PLILQHENLE1021TIVDFIKSTL PNLGLVQMEK TINQVFEMDI AKQLQAYEVE YHVLQEELID SSPLSDNQRM1081DKLEKTNSSL RKQNLDLLEQ LQVANGRIQS LEATIEKLLS SESKLKQAML TLELERSALL1141QTVEELRRRS AEPSDREPEC TQPEPTGDTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1168 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.