Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TBATA / C10orf27 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G106079-100
Catalog Number: LS-G106079
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SPATIAL(TBATA) (NM_152710), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- C10orf27 | Protein SPATIAL | Protein TBATA | Spatial | TBATA
- Origin Species
- Human
- SwissProt
- Q96M53
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 39.3 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MATDVQLAGY PLMSPKAELK LEKKSGRKPR SPRDSGPQKE LVIPGIVDFE RIRRALRTPK61PQTPGTYCFG RLSHHSFFSR HHPHPQHVTH IQDLTGKPVC VVRDFPAPLP ESTVFSGCQM121GIPTISVPIG DPQSNRNPQL SSEAWKKELK ELASRVAFLT KEDELKKKEK EQKEEPLREQ181GAKYSAETGR LIPASTRAVG RRRSHQGQQS QSSSRHEGVQ AFLLQDQELL VLELLCQILE241TDLLSAIQFW LLYAPPKEKD LALGLLQTAV AQLLPQPLVS IPTEKLLSQL PEVHEPPQEK301QEPPCSQSPK KTKISPFTKS EKPEYIGEAQ VLQMHSSQNT EKKTSKPRAE STRTRPLEQKLISEEDLAANDILDYKDDDDKV
351 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.