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TAX1BP1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TAX1BP1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G100703-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TAX1BP1 (NM_006024), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CALCOCO3 | T6BP | Tax1-binding protein 1 | TAX1BP1 | TRAF6-binding protein | TXBP151
Origin Species
Human
NCBI GenBank
NM_006024
SwissProt
Q86VP1

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
90.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MTSFQEVPLQ TSNFAHVIFQ NVAKSYLPNA HLECHYTLTP YIHPHPKDWV GIFKVGWSTA
61RDYYTFLWSP MPEHYVEGST VNCVLAFQGY YLPNDDGEFY QFCYVTHKGE IRGASTPFQF
121RASSPVEELL TMEDEGNSDM LVVTTKAGLL ELKIEKTMKE KEELLKLIAV LEKETAQLRE
181QVGRMERELN HEKERCDQLQ AEQKGLTEVT QSLKMENEEF KKRFSDATSK AHQLEEDIVS
241VTHKAIEKET ELDSLKDKLK KAQHEREQLE CQLKTEKDEK ELYKVHLKNT EIENTKLMSE
301VQTLKNIDGN KESVITHFKE EIGRLQLCLA EKENLQRTFL LTTSSKEDTC FLKEQLRKAE
361EQVQATRQEV VFLAKELSDA VNVRDRTMAD LHTARLENEK VKKQLADAVA ELKLNAMKKD
421QDKTDTLEHE LRREVEDLKL RLQMAADHYK EKFKECQRLQ KQINKLSDQS ANNNNVFTKK
481TGNQQKVNDA SVNTDPATSA STVDVKPSPS AAEADFDIVT KGQVCEMTKE IADKTEKYNK
541CKQLLQDEKA KCNKYADELA KMELKWKEQV KIAENVKLEL AEVQDNYKEL KRSLENPAER
601KMEGQNSQSP QCFKTCSEQN GYVLTLSNAQ PVLQYGNPYA SQETRDGADG AFYPDEIQRP
661PVRVPSWGLE DNVVCSQPAR NFSRPDGLED SEDSKEDENV PTAPDPPSQH LRGHGTGFCF
721DSSFDVHKKC PLCELMFPPN YDQSKFEEHV ESHWKVCPMC SEQFPPDYDQ QVFERHVQTH
781FDQNVLNFD
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

789 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.