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TATDN2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TATDN2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all TATDN2 antibodies →
$725.00each
Catalog Number: LS-G113725-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TATDN2 (NM_014760), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA0218 | TatD DNase domain containing 2 | TATDN2
Origin Species
Human
NCBI GenBank
D86972
SwissProt
Q93075

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
84.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MASERGKVKH NWSSTSEGCP RKRSCLREPC DVAPSSRPAQ RSASRSGGPS SPKRLKAQKE
61DDVACSRRLS WGSSRRRNNS SSSFSPHFLG PGVGGAASKG CLIRNTRGFL SSGGSPLRPA
121NASLEEMASL EEEACSLKVD SKDSSHNSTN SEFAAEAEGQ NDTIEEPNKV QKRKRDRLRD
181QGSTMIYLKA IQGILGKSMP KRKGEAATRA KPSAAEHPSH GEGPARSEGP AKTAEGAARS
241VTVTAAQKEK DATPEISMEE DKTVPERSSF YDRRVVIDPQ EKPSEEPLGD RRTVIDKCSP
301PLEFLDDSDS HLEIQKHKDR EVVMEHPSSG SDWSDVEEIS TVRFSQEEPV SLKPSAVPEP
361SSFTTDYVMY PPHLYSSPWC DYASYWTSSP KPSSYPSTGS SSNDAAQVGK SSRSRMSDYS
421PNSTGSVQNT SRDMEASEEG WSQNSRSFRF SRSSEEREVK EKRTFQEEMP PRPCGGHASS
481SLPKSHLEPS LEEGFIDTHC HLDMLYSKLS FQGTFTKFRK IYSSSFPKEF QGCISDFCDP
541RTLTDCLWEE LLKEDLVWGA FGCHPHFARY YSESQERNLL QALRHPKAVA FGEMGLDYSY
601KCTTPVPEQH KVFERQLQLA VSLKKPLVIH CREADEDLLE IMKKFVPPDY KIHRHCFTGS
661YPVIEPLLKY FPNMSVGFTA VLTYSSAWEA REALRQIPLE RIIVETDAPY FLPRQVPKSL
721CQYAHPGLAL HTVREIARVK DQPLSLTLAA LRENTSRLYS L
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

761 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.