Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TASP / LANCL2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G111572-100
Catalog Number: LS-G111572
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human LANCL2 (NM_018697), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- G protein-coupled receptor 69B | GPR69B | LanC-like protein 2 | LANCL2 | Tasp
- Origin Species
- Human
- NCBI GenBank
- NM_018697
- SwissProt
- Q9NS86
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 50.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGETMSKRLK LHLGGEAEME ERAFVNPFPD YEAAAGALLA SGAAEETGCV RPPATPDEPG61LPFHQDGKII HNFIRRIQTK IKDLLQQMEE GLKTADPHDC SAYTGWTGIA LLYLQLYRVT121CDQTYLLRSL DYVKRTLRNL NGRRVTFLCG DAGPLAVGAV IYHKLRSDCE SQECVTKLLQ181LQRSVVCQES DLPDELLYGR AGYLYALLYL NTEIGPGTVC ESAIKEVVNA IIESGKTLSR241EERKTERCPL LYQWHRKQYV GAAHGMAGIY YMLMQPAAKV DQETLTEMVK PSIDYVRHKK301FRSGNYPSSL SNETDRLVHW CHGAPGVIHM LMQAYKVFKE EKYLKEAMEC SDVIWQRGLL361RKGYGICHGT AGNGYSFLSL YRLTQDKKYL YRACKFAEWC LDYGAHGCRI PDRPYSLFEG421MAGAIHFLSD VLGPETSRFP AFELDSSKRDTRTRPLEQKLISEEDLAANDILDYKDDDDKV
450 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.