Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TAS2R16 / T2R16 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G112468-100
Catalog Number: LS-G112468
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TAS2R16 (NM_016945), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Candidate taste receptor T2R16 | T2R16 | TAS2R16
- Origin Species
- Human
- NCBI GenBank
- NM_016945
- SwissProt
- Q9NYV7
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 33.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MIPIQLTVFF MIIYVLESLT IIVQSSLIVA VLGREWLQVR RLMPVDMILI SLGISRFCLQ61WASMLNNFCS YFNLNYVLCN LTITWEFFNI LTFWLNSLLT VFYCIKVSSF THHIFLWLRW121RILRLFPWIL LGSLMITCVT IIPSAIGNYI QIQLLTMEHL PRNSTVTDKL ENFHQYQFQA181HTVALVIPFI LFLASTIFLM ASLTKQIQHH STGHCNPSMK AHFTALRSLA VLFIVFTSYF241LTILITIIGT LFDKRCWLWV WEAFVYAFIL MHSTSLMLSS PTLKRILKGK CTRTRPLEQKLISEEDLAANDILDYKDDDDKV
291 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.