Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TAPBPL / TAPBPR Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G112019-100
Catalog Number: LS-G112019
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Tapasin Related Protein(TAPBPL) (NM_018009), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- TAP binding protein related | TAPBP-R | TAPBPL | TAP binding protein-like | Tapasin-related protein | TAPASIN-R | TAPBPR | TAP-binding protein-like | Tapasin-like
- Origin Species
- Human
- NCBI GenBank
- NM_018009
- SwissProt
- Q9BX59
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 50 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGTQEGWCLL LCLALSGAAE TKPHPAEGQW RAVDVVLDCF LVKDGAHRGA LASSEDRARA61SLVLKQVPVL DDGSLEDFTD FQGGTLAQDD PPIIFEASVD LVQIPQAEAL LHADCSGKEV121TCEISRYFLQ MTETTVKTAA WFMANVQVSG GGPSISLVMK TPRVAKNEVL WHPTLNLPLS181PQGTVRTAVE FQVMTQTQSL SFLLGSSASL DCGFSMAPGL DLISVEWRLQ HKGRGQLVYS241WTAGQGQAVR KGATLEPAQL GMARDASLTL PGLTIQDEGT YICQITTSLY RAQQIIQLNI301QASPKVRLSL ANEALLPTLI CDIAGYYPLD VVVTWTREEL GGSPAQVSGA SFSSLRQSVA361GTYSISSSLT AEPGSAGATY TCQVTHISLE EPLGASTQVV PPERRTALGV IFASSLFLLA421LMFLGLQRRQ APTGLGLLQA ERWETTSCAD TQSSHLHEDR TARVSQPSTR TRPLEQKLIS481EEDLAANDIL DYKDDDDKV
499 aa
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.