Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TAOK2 / TAO2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G100387-100
Catalog Number: LS-G100387
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TAOK2 (NM_004783), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Kinase from chicken homolog C | HKFC-C | PSK | PSK-1 | PSK1-BETA | TAO1 | TAO kinase 2 | TAO2 | TAOK2 | PSK1 | KIAA0881 | MAP3K17
- Origin Species
- Human
- NCBI GenBank
- NM_004783
- SwissProt
- Q9UL54
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 119.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MPAGGRAGSL KDPDVAELFF KDDPEKLFSD LREIGHGSFG AVYFARDVRN SEVVAIKKMS61YSGKQSNEKW QDIIKEVRFL QKLRHPNTIQ YRGCYLREHT AWLVMEYCLG SASDLLEVHK121KPLQEVEIAA VTHGALQGLA YLHSHNMIHR DVKAGNILLS EPGLVKLGDF GSASIMAPAN181SFVGTPYWMA PEVILAMDEG QYDGKVDVWS LGITCIELAE RKPPLFNMNA MSALYHIAQN241ESPVLQSGHW SEYFRNFVDS CLQKIPQDRP TSEVLLKHRF VLRERPPTVI MDLIQRTKDA301VRELDNLQYR KMKKILFQEA PNGPGAEAPE EEEEAEPYMH RAGTLTSLES SHSVPSMSIS361ASSQSSSVNS LADASDNEEE EEEEEEEEEE EEGPEAREMA MMQEGEHTVT SHSSIIHRLP421GSDNLYDDPY QPEITPSPLQ PPAAPAPTST TSSARRRAYC RNRDHFATIR TASLVSRQIQ481EHEQDSALRE QLSGYKRMRR QHQKQLLALE SRLRGEREEH SARLQRELEA QRAGFGAEAE541KLARRHQAIG EKEARAAQAE ERKFQQHILG QQKKELAALL EAQKRTYKLR KEQLKEELQE601NPSTPKREKA EWLLRQKEQL QQCQAEEEAG LLRRQRQYFE LQCRQYKRKM LLARHSLDQD661LLREDLNKKQ TQKDLECALL LRQHEATREL ELRQLQAVQR TRAELTRLQH QTELGNQLEY721NKRREQELRQ KHAAQVRQQP KSLKSKELQI KKQFQETCKI QTRQYKALRA HLLETTPKAQ781HKSLLKRLKE EQTRKLAILA EQYDQSISEM LSSQALRLDE TQEAEFQALR QQLQQELELL841NAYQSKIKIR TESQHERELR ELEQRVALRR ALLEQRVEEE LLALQTGRSE RIRSLLERQA901REIEAFDAES MRLGFSSMAL GGIPAEAAAQ GYPAPPPAPA WPSRPVPRSG AHWSHGPPPP961GMPPPAWRQP SLLAPPGPPN WLGPPTQSGT PRGGALLLLR NSPQPLRRAA SGGSGSENVG1021PPAAAVPGPL SRSTSVASHI LNGSSHFYSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1049 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.