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TAGAP Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TAGAP Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all TAGAP antibodies →
$725.00each
Catalog Number: LS-G106441-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TAGAP (NM_152133), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
FKSG15 | IDDM21 | TAGAP1 | ARHGAP47 | TAGAP
Origin Species
Human
SwissProt
Q8N103

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
60.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGALEMQDEE DRIEALKQVA DKLPRPNLLL LKHLVYVLHL ISKNSEVNRM DSSNLAICIG
61PNMLTLENDQ SLSFEAQKDL NNKVKTLVEF LIDNCFEIFG ENIPVHSSIT SDDSLEHTDS
121SDVSTLQNDS AYDSNDPDVE SNSSSGISSP SRQPQVPMAT AAGLDSAGPQ DAREVSPEPI
181VSTVARLKSS LAQPDRRYSE PSMPSSQECL ESRVTNQTLT KSEGDFPVPR VGSRLESEEA
241EDPFPEEVFP AVQGKTKRPV DLKIKNLAPG SVLPRALVLK AFSSSSLDAS SDSSPVASPS
301SPKRNFFSRH QSFTTKTEKG KPSREIKKHS MSFTFAPHKK VLTKNLSAGS GKSQDFTRDH
361VPRGVRKESQ LAGRIVQENG CETHNQTARG FCLRPHALSV DDVFQGADWE RPGSPPSYEE
421AMQGPAARLV ASESQTVGSM TVGSMRARML EAHCLLPPLP PAHHVEDSRH RGSKEPLPGH
481GLSPLPERWK QSRTVHASGD SLGHVSGPGR PELLPLRTVS ESVQRNKRDC LVRRCSQPVF
541EADQFQYAKE SYI
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

553 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.