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TAF4B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TAF4B Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all TAF4B antibodies →
$725.00each
Catalog Number: LS-G115834-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TAF4B (NM_005640), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
TAF(II)105 | TAFII105 | TAF2C2 | TAF4B | TAFII-105
Origin Species
Human
NCBI GenBank
Y09321
SwissProt
Q92750

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
90.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MPAGLTEPAG AAPPAAVSAS GTVTMAPAGA LPVRVESTPV ALGAVTKAPV SVCVEPTASQ
61PLRSPVGTLV TKVAPVSAPP KVSSGPRLPA PQIVAVKAPN TTTIQFPANL QLPPGTVLIK
121SNSGPLMLVS PQQTVTRAET TSNITSRPAV PANPQTVKIC TVPNSSSQLI KKVAVTPVKK
181LAQIGTTVVT TVPKPSSVQS VAVPTSVVTV TPGKPLNTVT TLKPSSLGAS STPSNEPNLK
241AENSAAVQIN LSPTMLENVK KCKNFLAMLI KLACSGSQSP EMGQNVKKLV EQLLDAKIEA
301EEFTRKLYVE LKSSPQPHLV PFLKKSVVAL RQLLPNSQSF IQQCVQQTSS DMVIATCTTT
361VTTSPVVTTT VSSSQSEKSI IVSGATAPRT VSVQTLNPLA GPVGAKAGVV TLHSVGPTAA
421TGGTTAGTGL LQTSKPLVTS VANTVTTVSL QPEKPVVSGT AVTLSLPAVT FGETSGAAIC
481LPSVKPVVSS AGTTSDKPVI GTPVQIKLAQ PGPVLSQPAG IPQAVQVKQL VVQQPSGGNE
541KQVTTISHSS TLTIQKCGQK TMPVNTIIPT SQFPPASILK QITLPGNKIL SLQASPTQKN
601RIKENVTSCF RDEDDINDVT SMAGVNLNEE NACILATNSE LVGTLIQSCK DEPFLFIGAL
661QKRILDIGKK HDITELNSDA VNLISQATQE RLRGLLEKLT AIAQHRMTTY KASENYILCS
721DTRSQLKFLE KLDQLEKQRK DLEEREMLLK AAKSRSNKED PEQLRLKQKA KELQQLELAQ
781IQHRDANLTA LAAIGPRKKR PLESGIEGLK DNLLASGTSS LTATKQLHRP RITRICLRDL
841IFCMEQEREM KYSRALYLAL LK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

862 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.