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TAF2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TAF2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all TAF2 antibodies →
$725.00each
Catalog Number: LS-G117482-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TAF2 (NM_003184), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CIF150 | TAF2 | TAF2B | TAFII-150 | TBP-associated factor 150 kDa | TAF(II)150 | TAFII150
Origin Species
Human
NCBI GenBank
NM_003184
SwissProt
Q6P1X5

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
136.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MPLTGVEPAR MNRKKGDKGF ESPRPYKLTH QVVCINNINF QRKSVVGFVE LTIFPTVANL
61NRIKLNSKQC RIYRVRINDL EAAFIYNDPT LEVCHSESKQ RNLNYFSNAY AAAVSAVDPD
121AGNGELCIKV PSELWKHVDE LKVLKIHINF SLDQPKGGLH FVVPSVEGSM AERGAHVFSC
181GYQNSTRFWF PCVDSYSELC TWKLEFTVDA AMVAVSNGDL VETVYTHDMR KKTFHYMLTI
241PTAASNISLA IGPFEILVDP YMHEVTHFCL PQLLPLLKHT TSYLHEVFEF YEEILTCRYP
301YSCFKTVFID EAYVEVAAYA SMSIFSTNLL HSAMIIDETP LTRRCLAQSL AQQFFGCFIS
361RMSWSDEWVL KGISGYIYGL WMKKTFGVNE YRHWIKEELD KIVAYELKTG GVLLHPIFGG
421GKEKDNPASH LHFSIKHPHT LSWEYYTMFQ CKAHLVMRLI ENRISMEFML QVFNKLLSLA
481STASSQKFQS HMWSQMLVST SGFLKSISNV SGKDIQPLIK QWVDQSGVVK FYGSFAFNRK
541RNVLELEIKQ DYTSPGTQKY VGPLKVTVQE LDGSFNHTLQ IEENSLKHDI PCHSKSRRNK
601KKKIPLMNGE EVDMDLSAMD ADSPLLWIRI DPDMSVLRKV EFEQADFMWQ YQLRYERDVV
661AQQESILALE KFPTPASRLA LTDILEQEQC FYRVRMSACF CLAKIANSMV STWTGPPAMK
721SLFTRMFCCK SCPNIVKTNN FMSFQSYFLQ KTMPVAMALL RDVHNLCPKE VLTFILDLIK
781YNDNRKNKFS DNYYRAEMID ALANSVTPAV SVNNEVRTLD NLNPDVRLIL EEITRFLNME
841KLLPSYRHTI TVSCLRAIRV LQKNGHVPSD PALFKSYAEY GHFVDIRIAA LEAVVDYTKV
901DRSYEELQWL LNMIQNDPVP YVRHKILNML TKNPPFTKNM ESPLCNEALV DQLWKLMNSG
961TSHDWRLRCG AVDLYFTLFG LSRPSCLPLP ELGLVLNLKE KKAVLNPTII PESVAGNQEA
1021ANNPSSHPQL VGFQNPFSSS QDEEEIDMDT VHDSQAFISH HLNMLERPST PGLSKYRPAS
1081SRSALIPQHS AGCDSTPTTK PQWSLELARK GTGKEQAPLE MSMHPAASAP LSVFTKESTA
1141SKHSDHHHHH HHEHKKKKKK HKHKHKHKHK HDSKEKDKEP FTFSSPASGR SIRSPSLSD
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1199 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.