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TAF1L Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TAF1L Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all TAF1L antibodies →
$725.00each
Catalog Number: LS-G105689-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TAF1L (NM_153809), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
TBP-associated factor 210 kDa | TAF(II)210 | TAF1L | TAF2A2 | TBP-associated factor 1-like
Origin Species
Human
NCBI GenBank
NM_153809
SwissProt
Q8IZX4

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
207.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MRPGCDLLLR AAATVTAAIM SDSDSEEDSS GGGPFTLAGI LFGNISGAGQ LEGESVLDDE
61CKKHLAGLGA LGLGSLITEL TANEELTGTG GALVNDEGWI RSTEDAVDYS DINEVAEDES
121QRHQQTMGSL QPLYHSDYDE DDYDADCEDI DCKLMPPPPP PPGPMKKDKD QDAITCVSES
181GEDIILPSII APSFLASEKV DFSSYSDSES EMGPQEATQA ESEDGKLTLP LAGIMQHDAT
241KLLPSVTELF PEFRPGKVLR FLHLFGPGKN VPSVWRSARR KRKKHRELIQ EEQIQEVECS
301VESEVSQKSL WNYDYAPPPP PEQCLADDEI TMMVPVESKF SQSTGDVDKV TDTKPRVAEW
361RYGPARLWYD MLGVSEDGSG FDYGFKLRKT QHEPVIKSRM MEEFRKLEES NGTDLLADEN
421FLMVTQLHWE DSIIWDGEDI KHKGTKPQGA SLAGWLPSIK TRNVMAYNVQ QGFAPTLDDD
481KPWYSIFPID NEDLVYGRWE DNIIWDAQAM PRLLEPPVLA LDPNDENLIL EIPDEKEEAT
541SNSPSKESKK ESSLKKSRIL LGKTGVIREE PQQNMSQPEV KDPWNLSNDE YYFPKQQGLR
601GTFGGNIIQH SIPAMELWQP FFPTHMGPIK IRQFHRPPLK KYSFGALSQP GPHSVQPLLK
661HIKKKAKMRE QERQASGGGE LFFMRTPQDL TGKDGDLILA EYSEENGPLM MQVGMATKIK
721NYYKRKPGKD PGAPDCKYGE TVYCHTSPFL GSLHPGQLLQ ALENNLFRAP VYLHKMPETD
781FLIIRTRQGY YIRELVDIFV VGQQCPLFEV PGPNSRRANM HIRDFLQVFI YRLFWKSKDR
841PRRIRMEDIK KAFPSHSESS IRKRLKLCAD FKRTGMDSNW WVLKSDFRLP TEEEIRAKVS
901PEQCCAYYSM IAAKQRLKDA GYGEKSFFAP EEENEEDFQM KIDDEVHAAP WNTTRAFIAA
961MKGKCLLEVT GVADPTGCGE GFSYVKIPNK PTQQKDDKEP QAVKKTVTGT DADLRRLSLK
1021NAKQLLRKFG VPEEEIKKLS RWEVIDVVRT MSTEQAHSGE GPMSKFARGS RFSVAEHQER
1081YKEECQRIFD LQNKVLSSTE VLSTDTDSIS AEDSDFEEMG KNIENMLQNK KTSSQLSREW
1141EEQERKELRR MLLVAGSAAS GNNHRDDVTA SMTSLKSSAT GHCLKIYRTF RDEEGKEYVR
1201CETVRKPAVI DAYVRIRTTK DEKFIQKFAL FDEKHREEMR KERRRIQEQL RRLKRNQEKE
1261KLKGPPEKKP KKMKERPDLK LKCGACGAIG HMRTNKFCPL YYQTNVPPSK PVAMTEEQEE
1321ELEKTVIHND NEELIKVEGT KIVFGKQLIE NVHEVRRKSL VLKFPKQQLP PKKKRRVGTT
1381VHCDYLNIPH KSIHRRRTDP MVTLSSILES IINDMRDLPN THPFHTPVNA KVVKDYYKII
1441TRPMDLQTLR ENVRKCLYPS REEFREHLEL IVKNSATYNG PKHSLTQISQ SMLDLCDEKL
1501KEKEDKLARL EKAINPLLDD DDQVAFSFIL DNIVTQKMMA VPDSWPFHHP VNKKFVPDYY
1561KMIVNPVDLE TIRKNISKHK YQSRESFLDD VNLILANSVK YNGPESQYTK TAQEIVNICY
1621QTITEYDEHL TQLEKDICTA KEAALEEAEL ESLDPMTPGP YTSQPPDMYD TNTSLSTSRD
1681ASVFQDESNL SVLDISTATP EKQMCQGQGR LGEEDSDVDV EGYDDEEEDG KPKPPAPEGG
1741DGDLADEEEG TVQQPEASVL YEDLLISEGE DDEEDAGSDE EGDNPFSAIQ LSESGSDSDV
1801GYGGIRPKQP FMLQHASGEH KDGHGK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1826 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.