Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TADA1 / ADA1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G108017-100
Catalog Number: LS-G108017
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TADA1L(TADA1) (NM_053053), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ADA1 | HFI1 | HADA1 | RP1-9E21.4 | TADA1 | TADA1L | SPT3-associated factor 42 | STAF42 | Transcriptional adapter 1 | Transcriptional adaptor 1
- Origin Species
- Human
- NCBI GenBank
- R54280
- SwissProt
- Q96BN2
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 37.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MATFVSELEA AKKNLSEALG DNVKQYWANL KLWFKQKISK EEFDLEAHRL LTQDNVHSHN61DFLLAILTRC QILVSTPDGA GSLPWPGGSA AKPGKPKGKK KLSSVRQKFD HRFQPQNPLS121GAQQFVAKDP QDDDDLKLCS HTMMLPTRGQ LEGRMIVTAY EHGLDNVTEE AVSAVVYAVE181NHLKDILTSV VSRRKAYRLR DGHFKYAFGS NVTPQPYLKN SVVAYNNLIE SPPAFTAPCA241GQNPASHPPP DDAEQQAALL LACSGDTLPA SLPPVNMYDL FEALQVHREV IPTHTVYALN301IERIITKLWH PNHEELQQDK VHRQRLAAKE GLLLCSGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
335 aa + 34 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.