Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
T2R5 / TAS2R5 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G111503-100
Catalog Number: LS-G111503
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TAS2R5 (NM_018980), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- TAS2R5 | T2R5 | Taste receptor type 2 member 5
- Origin Species
- Human
- NCBI GenBank
- NM_018980
- SwissProt
- Q9NYW4
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 34.3 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MLSAGLGLLM LVAVVEFLIG LIGNGSLVVW SFREWIRKFN WSSYNLIILG LAGCRFLLQW61LIILDLSLFP LFQSSRWLRY LSIFWVLVSQ ASLWFATFLS VFYCKKITTF DRPAYLWLKQ121RAYNLSLWCL LGYFIINLLL TVQIGLTFYH PPQGNSSIRY PFESWQYLYA FQLNSGSYLP181LVVFLVSSGM LIVSLYTHHK KMKVHSAGRR DVRAKAHITA LKSLGCFLLL HLVYIMASPF241SITSKTYPPD LTSVFIWETL MAAYPSLHSL ILIMGIPRVK QTCQKILWKA VCARRCWGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
299 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.