Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
T2R42 / TAS2R42 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G104503-100
Catalog Number: LS-G104503
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TAS2R42 (NM_181429), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- HT2R55 | T2R24 | T2R55 | TAS2R42 | T2R42 | TAS2R55 | Taste receptor T2R55
- Origin Species
- Human
- SwissProt
- Q7RTR8
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 36 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MATELDKIFL ILAIAEFIIS MLGNVFIGLV NCSEGIKNQK VFSADFILTC LAISTIGQLL61VILFDSFLVG LASHLYTTYR LGKTVIMLWH MTNHLTTWLA TCLSIFYFFK IAHFPHSLFL121WLRWRMNGMI VMLLILSLFL LIFDSLVLEI FIDISLNIID KSNLTLYLDE SKTLYDKLSI181LKTLLSLTSF IPFSLFLTSL LFLFLSLVRH TRNLKLSSLG SRDSSTEAHR RAMKMVMSFL241FLFIVHFFSL QVANGIFFML WNNKYIKFVM LALNAFPSCH SFILILGNSK LRQTAVRLLW301HLRNYTKTPN ALPLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
314 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.