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SYTL5 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SYTL5 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G107229-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SYTL5 (NM_138780), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Exophilin 9 | Synaptotagmin-like protein 5 | SYTL5 | Synaptotagmin-like 5 | Slp5
Origin Species
Human
SwissProt
Q8TDW5

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
81.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSKNSEFINL SFLLDHEKEM ILGVLKRDEY LKKVEDKRIR KLKNELLEAK RRSGKTQQEA
61SRVCVHCHRN LGLIFDRGDP CQACSLRVCR ECRVAGPNGS WKCTVCDKIA QLRIITGEWF
121FEEKAKRFKQ VNVLGTDVVR QSILRRSPGA EEVQSQEQTR QDAEKSDTSP VAGKKASHDG
181PKRKGFLLSK FRSATRGEII TPKTDTGRSY SLDLDGQHFR SLKSPPGSDR GSTGSSDLND
241QEPGPRTPKS SRSNGVTPGT QSSPAPSTRT VTSVISREYG FENSMDLAAI EGTSQELTKS
301HRRNTSGTPS IAVSGTSLSS DQSRSELDLS ESFTEDSEDT VSIRSKSVPG ALDKDSLEET
361EESIDALVSS QLSTNTHRLA SGLSTTSLNS MMSVYSETGD YGNVKVSGEI LLHISYCYKT
421GGLYIFVKNC RNLAIGDEKK QRTDAYVKSY LLPDKSRNNK RKTKIRTGTN PEFNETLKYT
481ISHTQLETRT LQLSVWHYDR FGRNSFLGEV EIPFDSWNFE NPTDEWFVLQ PKVEFAPDIG
541LQYKGELTVV LRYIPPEENL MLPPEQLQGN KTFKKGKKKE SPVISGGILE VFIKEAKNLT
601AVKSGGTSDS FVKGYLLPDD SKATKHKTLV IKKSVNPQWN HTFMFSGIHP QDIKNVCLEL
661TIWDKEAFSS NIFLGGVRLN SGSGVSHGKN VDWMDSQGEE QRLWQKMANN PGTPFEGVLM
721LRSSMGKCRL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

730 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.