Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SYTL4 / Granuphilin Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G124923-100
Catalog Number: LS-G124923
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Granuphilin(SYTL4) (NM_001129896), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Exophilin-2 | Granuphilin | Synaptotagmin-like 4 | Granuphilin-a | SLP4 | Synaptotagmin-like protein 4 | SYTL4
- Origin Species
- Human
- SwissProt
- Q96C24
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 75.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSELLDLSFL SEEEKDLILS VLQRDEEVRK ADEKRIRRLK NELLEIKRKG AKRGSQHYSD61RTCARCQESL GRLSPKTNTC RGCNHLVCRD CRIQESNGTW RCKVCAKEIE LKKATGDWFY121DQKVNRFAYR TGSEIIRMSL RHKPAVSKRE TVGQSLLHQT QMGDIWPGRK IIQERQKEPS181VLFEVPKLKS GKSALEAESE SLDSFTADSD STSRRDSLDK SGLFPEWKKM SAPKSQVEKE241TQPGGQNVVF VDEGEMIFKK NTRKILRPSE YTKSVIDLRP EDVVHESGSL GDRSKSVPGL301NVDMEEEEEE EDIDHLVKLH RQKLARSSMQ SGSSMSTIGS MMSIYSEAGD FGNIFVTGRI361AFSLKYEQQT QSLVVHVKEC HQLAYADEAK KRSNPYVKTY LLPDKSRQGK RKTSIKRDTI421NPLYDETLRY EIPESLLAQR TLQFSVWHHG RFGRNTFLGE AEIQMDSWKL DKKLDHCLPL481HGKISAESPT GLPSHKGELV VSLKYIPASK TPVGGDRKKS KGGEGGELQV WIKEAKNLTA541AKAGGTSDSF VKGYLLPMRN KASKRKTPVM KKTLNPHYNH TFVYNGVRLE DLQHMCLELT601VWDREPLASN DFLGGVRLGV GTGISNGEVV DWMDSTGEEV SLWQKMRQYP GSWAEGTLQL661RSSMAKQKLG LTRTRPLEQKLISEEDLAANDILDYKDDDDKV
671 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.