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SYTL3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SYTL3 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G121678-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SYTL3 (NM_001009991), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Exophilin-6 | SLP3 | Synaptotagmin-like 3 | SYTL3 | Synaptotagmin-like protein 3
Origin Species
Human
NCBI GenBank
NM_001009991
SwissProt
Q4VX76

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
60.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAQEIDLSAL KELEREAILQ VLYRDQAVQN TEEERTRKLK THLQHLRWKG AKNTDWEHKE
61KCCARCQQVL GFLLHRGAVC RGCSHRVCAQ CRVFLRGTHA WKCTVCFEDR NVKIKTGEWF
121YEERAKKFPT GGKHETVGGQ LLQSYQKLSK ISVVPPTPPP VSESQCSRSP GRKVSAPDIL
181KPLNQEDPKC STNPILKQQN LPSSPAPSTI FSGGFRHGSL ISIDSTCTEM GNFDNANVTG
241EIEFAIHYCF KTHSLEICIK ACKNLAYGEE KKKKCNPYVK TYLLPDRSSQ GKRKTGVQRN
301TVDPTFQETL KYQVAPAQLV TRQLQVSVWH LGTLARRVFL GEVIIPLATW DFEDSTTQSF
361RWHPLRAKAE KYEDSVPQSN GELTVRAKLV LPSRPRKLQE AQEGTDQPSL HGQLCLVVLG
421AKNLPVRPDG TLNSFVKGCL TLPDQQKLRL KSPVLRKQAC PQWKHSFVFS GVTPAQLRQS
481SLELTVWDQA LFGMNDRLLG GTRLGSKGDT AVGGDACSQS KLQWQKVLSS PNLWTDMTLV
541LH
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

542 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.