Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SYTL2 / SLP2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G102958-100
Catalog Number: LS-G102958
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SYTL2 (NM_206929), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CHR11SYT | EXO4 | Exophilin-4 | KIAA1597 | SGA72M | SLP2 | Synaptotagmin-like protein 2 | Synaptotagmin-like 2 | SLP2A | SYTL2 | Chromosome 11 synaptotagmin
- Origin Species
- Human
- NCBI GenBank
- NM_032943
- SwissProt
- Q9HCH5
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 42.6 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSKSVPAFLQ DESDDRETDT ASESSYQLSR HKKSPSSLTN LSSSSGMTSL SSVSGSVMSV61YSGDFGNLEV KGNIQFAIEY VESLKELHVF VAQCKDLAAA DVKKQRSDPY VKAYLLPDKG121KMGKKKTLVV KKTLNPVYNE ILRYKIEKQI LKTQKLNLSI WHRDTFKRNS FLGEVELDLE181TWDWDNKQNK QLRWYPLKRK TAPVALEAEN RGEMKLALQY VPEPVPGKKL PTTGEVHIWV241KECLDLPLLR GSHLNSFVKC TILPDTSRKS RQKTRAVGKT TNPIFNHTMV YDGFRPEDLM301EACVELTVWD HYKLTNQFLG GLRIGFGTGK SYGTEVDWMD STSEEVALWE KMVNSPNTWI361EATLPLRMLL IAKISKTRTRPLEQKLISEEDLAANDILDYKDDDDKV
376 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.