Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SYTL2 / SLP2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G102089-100
Catalog Number: LS-G102089
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SYTL2 (NM_032943), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CHR11SYT | EXO4 | Exophilin-4 | KIAA1597 | SGA72M | SLP2 | Synaptotagmin-like protein 2 | Synaptotagmin-like 2 | SLP2A | SYTL2 | Chromosome 11 synaptotagmin
- Origin Species
- Human
- NCBI GenBank
- NM_032943
- SwissProt
- Q9HCH5
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 102.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MIDLSFLTEE EQEAIMKVLQ RDAALKRAEE ERVRHLPEKI KDDQQLKNMS GQWFYEAKAK61RHRDKIHGAD IIRASMRKKR PQIAAEQSKD RENGAKESWV NNVNKDAFLP PELAGVVEEP121EEDAAPASPS SSVVNPASSV IDMSQENTRK PNVSPEKRKN PFNSSKLPEG HSSQQTKNEQ181SKNGRTGLFQ TSKEDELSES KEKSTVADTS IQKLEKSKQT LPGLSNGSQI KAPIPKARKM241IYKSTDLNKD DNQSFPRQRT DSLKARGAPR GILKRNSSSS STDSETLRYN HNFEPKSKIV301SPGLTIHERI SEKEHSLEDN SSPNSLEPLK HVRFSAVKDE LPQSPGLIHG REVGEFSVLE361SDRLKNGMED AGDTEEFQSD PKPSQYRKPS LFHQSTSSPY VSKSETHQPM TSGSFPINGL421HSHSEVLTAR PQSMENSPTI NEPKDKSSEL TRLESVLPRS PADELSHCVE PEPSQVPGGS481SRDRQQGSEE EPSPVLKTLE RSAARKMPSK SLEDISSDSS NQAKVDNQPE ELVRSAEDDE541KPDQKPVTNE CVPRISTVPT QPDNPFSHPD KLKRMSKSVP AFLQDEVSGS VMSVYSGDFG601NLEVKGNIQF AIEYVESLKE LHVFVAQCKD LAAADVKKQR SDPYVKAYLL PDKGKMGKKK661TLVVKKTLNP VYNEILRYKI EKQILKTQKL NLSIWHRDTF KRNSFLGEVE LDLETWDWDN721KQNKQLRWYP LKRKTAPVAL EAENRGEMKL ALQYVPEPVP GKKLPTTGEV HIWVKECLDL781PLLRGSHLNS FVKCTILPDT SRKSRQKTRA VGKTTNPIFN HTMVYDGFRP EDLMEACVEL841TVWDHYKLTN QFLGGLRIGF GTGKSYGTEV DWMDSTSEEV ALWEKMVNSP NTWIEATLPL901RMLLIAKISKTRTRPLEQKLISEEDLAANDILDYKDDDDKV
910 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.