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SYTL1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SYTL1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G108600-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SYTL1 (NM_032872), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Exophilin-7 | JFC1 | SLP1 | Synaptotagmin-like 1 | Synaptotagmin-like protein 1 | SYTL1 | Protein JFC1
Origin Species
Human
NCBI GenBank
NM_032872
SwissProt
Q8IYJ3

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
60.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MPQRGHPSQE GLWALPSLPM AHGPKPETEG LLDLSFLTEE EQEAIAGVLQ RDARLRQLEE
61GRVSKLRASV ADPGQLKILT GDWFQEARSQ RHHNAHFGSD LVRASMRRKK STRGDQAPGH
121DREAEAAVKE KEEGPEPRLT IDEAPQERLR ETEASDPEEA SQAQEDPGQG DQQVCAEEAD
181PELEPASGGE QEPRPQQAQT KAASQILENG EEAPGPDPSL DRMLSSSSSV SSLNSSTLSG
241SQMSLSGDAE AVQVRGSVHF ALHYEPGAAE LRVHVIQCQG LAAARRRRSD PYVKSYLLPD
301KQSKRKTAVK KRNLNPVFNE TLRYSVPQAE LQGRVLSLSV WHRESLGRNI FLGEVEVPLD
361TWDWGSEPTW LPLQPRVPPS PDDLPSRGLL ALSLKYVPAG SEGAGLPPSG ELHFWVKEAR
421DLLPLRAGSL DTYVQCFVLP DDSQASRQRT RVVRRSLSPV FNHTMVYDGF GPADLRQACA
481ELSLWDHGAL ANRQLGGTRL SLGTGSSYGL QVPWMDSTPE EKQLWQALLE QPCEWVDGLL
541PLRTNLAPRT
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

550 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.