Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SYT9 / Synaptotagmin 9 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G104985-100
Catalog Number: LS-G104985
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SYT9 (NM_175733), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Synaptotagmin IX | Synaptotagmin-9 | SytIX | SYT9
- Origin Species
- Human
- SwissProt
- Q86SS6
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 56 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MPGARDALCH QALQLLAELC ARGALEHDSC QDFIYHLRDR ARPRLRDPDI SVSLLTLVVT61ACGLALFGVS LFVSWKLCWV PWRERGLPSG SKDNNQEPLN YMDTETNEQE NSEDFLDPPT121PCPDSSMKIS HTSPDIPLST QTGIQENCAH GVRVQRQVTE PTSSARHNSI RRQLNLSNPD181FNIQQLQKQE QLTGIGRIKP ELYKQRSLDN DDGRRSNSKA CGKLNFILKY DCDLEQLIVK241IHKAVNLPAK DFSGTSDPYV KIYLLPDRKT KHQTKVHRKT LNPVFDEVFL FPVPYNDLEA301RKLHFSVYDF DRFSRHDLIG QVVVDHFLDL ADFPRECILW KDIEYVTNDN VDLGELMFSL361CYLPTAGRLT ITIIKARNLK AMDITGASDP YVKVSLMCDG RRLKKRKTST KRNTLNPVYN421EAIVFDVPPE NIDQIHLSIA VMDYDRVGHN EIIGVCQVGN EAERLGRDHW SEMLSYPRKP481IAHWHSLVEK RTRTRPLEQKLISEEDLAANDILDYKDDDDKV
491 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.