Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SYT6 / Synaptotagmin 6 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G102979-100
Catalog Number: LS-G102979
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Synaptotagmin VI(SYT6) (NM_205848), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Synaptotagmin VI | SytVI | Synaptotagmin-6 | SYT6
- Origin Species
- Human
- SwissProt
- Q5T7P8
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 48.3 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MPWRNKEASS PSSANPPLEA LQSPSFRGNM ADKLKDPSTL GFLEAAVKIS HTSPDIPAEV61QMSVKEHIMR HTRLQRQTTE PASSTRHTSF KRHLPRQMHV SSVDYGNELP PAAEQPTSIG121RIKPELYKQK SVDGEDAKSE ATKSCGKINF SLRYDYETET LIVRILKAFD LPAKDFCGSS181DPYVKIYLLP DRKCKLQTRV HRKTLNPTFD ENFHFPVPYE ELADRKLHLS VFDFDRFSRH241DMIGEVILDN LFEASDLSRE TSIWKDIQYA TSESVDLGEI MFSLCYLPTA GRLTLTVIKC301RNLKAMDITG YSDPYVKVSL LCDGRRLKKK KTTIKKNTLN PVYNEAIIFD IPPENMDQVS361LLISVMDYDR VGHNEIIGVC RVGITAEGLG RDHWNEMLAY PRKPIAHWHS LVEVKKSFKE421GNPRLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
425 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.