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SYT16 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SYT16 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all SYT16 antibodies →
$504.00each
Catalog Number: LS-G109137-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SYT16 (NM_031914), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Chr14 synaptotagmin | CHR14SYT | Syt14r | SYT16 | Synaptotagmin-16 | Synaptotagmin XVI | Strep14 | Synaptotagmin 14-like protein | Synaptotagmin XIV-like | SYT14L | Yt14r
Origin Species
Human
SwissProt
Q17RD7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
72.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MVLAMASQDA QNFFQPFSSW ISRVYEALQQ AGDMLSASLV NISKQDSKLS DKLDQDLDNI
61QIQETYFEDE EQDNDWSQED ANSLFLEVDH FSCCNSDLQD SAQNSSPSLS QHAKDSCSTM
121SQWPNWASDD RKLPHVLSSI AEEEHHLEKQ RSGLQHGFDS QLPGTLETVN GKKQVNSFGD
181DEELSTSSDS DEEVIKQFEI SVSRSQSFRS VTSEKGKQTG LEQKPKFSRS LLTHGEDGTE
241VSACEDLDGA SQRRYSENLS YGEDDHIPAH SQSPCERGDA KHHGTSHQES SVVQSLRRQS
301TEGSLEMETA FNSRGFEDSY ATDSSSMWSP EEQDRTNLQV PSGVSEPISK CGDLDVIFEY
361RAASQKLTVT IVRAQGLPDK DRSGVNSWQV HVVLLPGKKH RGRTNIQRGP NPVFREKVTF
421AKLEPRDVAA CAVRFRLYAA RKMTRERMMG EKLFYLSHLH PEGEMKVTLV LEPRSNISSG
481GSPLSPSAVS HSDSTSSTQS LSHGGAPELL VGLSYNATTG RLSVEMIKGS HFRNLAVNRA
541PDTYGKLFLL NSVGQEMSRC KTSIRRGQPN PVYKETFVFQ VALFQLSDVT LMISVYNRRT
601MKRKEMIGWI ALGQNSSGEE EQDHWEEMKE TKGQQICRWH TLLES
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

645 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.