Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SYT12 / Synaptotagmin12 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G104802-100
Catalog Number: LS-G104802
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SYT12 (NM_177963), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- SRG1 | Synaptotagmin-12 | Synaptotagmin-XII | Synaptotagmin XII | SYT12 | SytXII
- Origin Species
- Human
- SwissProt
- Q8IV01
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 46.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAVDVAEYHL SVIKSPPGWE VGVYAAGALA LLGIAAVSLW KLWTSGSFPS PSPFPNYDYR61YLQQKYGESC AEAREKRVPA WNAQRASTRG PPSRKGSLSI EDTFESISEL GPLELMGREL121DLAPYGTLRK SQSADSLNSI SSVSNTFGQD FTLGQVEVSM EYDTASHTLN VAVMQGKDLL181EREEASFESC FMRVSLLPDE QIVGISRIQR NAYSIFFDEK FSIPLDPTAL EEKSLRFSVF241GIDEDERNVS TGVVELKLSV LDLPLQPFSG WLYLQDQNKA ADAVGEILLS LSYLPTAERL301TVVVVKAKNL IWTNDKTTAD PFVKVYLLQD GRKMSKKKTA VKRDDPNPVF NEAMIFSVPA361IVLQDLSLRV TVAESSSDGR GDNVGHVIIG PSASGMGTTH WNQMLATLRR PVSMWHAVRR421NTRTRPLEQKLISEEDLAANDILDYKDDDDKV
421 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.