Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SYT10 / Synaptotagmin X Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G103475-100
Catalog Number: LS-G103475
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SYT10 (NM_198992), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Synaptotagmin X | Synaptotagmin-10 | SytX | Synaptotagmin 10 | SYT10
- Origin Species
- Human
- SwissProt
- Q6XYQ8
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 58.9 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MSFHKEDGVN SLCQKALHIV TELCFAGQVE WEKCSGIFPR DRGSQGGSST DISVSLLAVV61VSFCGLALLV VSLFVFWKLC WPCWKSKPVT SNITTLPQSI SSAPTEVFET EEKKEIKENE121KPAVKAIEPA IKISHTSPDI PAEVQTALKE HLIKHARVQR QITEPTSSTR HSSFRRHLPR181QMQVSSVDFS MGTEPVLQRG ETTTSIGRIK PELYKQKSVD SEGNQNEDVK ICGKLNFTLQ241YDYENELLVV KIIKALDLPA KDFTGTSDPY VKMYLLPDRK KKFQTRVHRK TLNPLFDETF301QFPVAYDQLS NRKLHFSVYD FDRFSRHDMI GEVILDNLFE VSDLSREATV WKDIHCATTE361SIDLGEIMFS LCYLPTAGRM TLTVIKCRNL KAMDITGSSD PYVKVSLMCE GRRLKKRKTT421TKKNTLNPVY NEAIIFDIPP ENVDQVSLSI AVMDYDRVGH NEVIGVCRTG LDAEGLGRDH481WNEMLAYHRK PITHWHPLLE LPGRATSFDS QGSCPSPKPP STPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
523 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.