Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SYNJ2 / Synaptojanin 2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G117022-100
Catalog Number: LS-G117022
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Synaptojanin 2(SYNJ2) (NM_003898), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- INPP5H | KIAA0348 | Synaptojanin-2 | SYNJ2 | Synaptojanin 2
- Origin Species
- Human
- NCBI GenBank
- AB002346
- SwissProt
- O15056
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 165.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MALSKGLRLL GRLGAEGDCS VLLEARGRDD CLLFEAGTVA TLAPEEKEVI KGQYGKLTDA61YGCLGELRLK SGGTSLSFLV LVTGCTSVGR IPDAEIYKIT ATDFYPLQEE AKEEERLIAL121KKILSSGVFY FSWPNDGSRF DLTVRTQKQG DDSSEWGNSF FWNQLLHVPL RQHQVSCCDW181LLKIICGVVT IRTVYASHKQ AKACLVSRVS CERTGTRFHT RGVNDDGHVS NFVETEQMIY241MDDGVSSFVQ IRGSVPLFWE QPGLQVGSHH LRLHRGLEAN APAFDRHMVL LKEQYGQQVV301VNLLGSRGGE EVLNRAFKKL LWASCHAGDT PMINFDFHQF AKGGKLEKLE TLLRPQLKLH361WEDFDVFTKG ENVSPRFQKG TLRMNCLDCL DRTNTVQSFI ALEVLHLQLK TLGLSSKPIV421DRFVESFKAM WSLNGHSLSK VFTGSRALEG KAKVGKLKDG ARSMSRTIQS NFFDGVKQEA481IKLLLVGDVY GEEVADKGGM LLDSTALLVT PRILKAMTER QSEFTNFKRI RIAMGTWNVN541GGKQFRSNVL RTAELTDWLL DSPQLSGATD SQDDSSPADI FAVGFEEMVE LSAGNIVNAS601TTNKKMWGEQ LQKAISRSHR YILLTSAQLV GVCLYIFVRP YHVPFIRDVA IDTVKTGMGG661KAGNKGAVGI RFQFHSTSFC FICSHLTAGQ SQVKERNEDY KEITQKLCFP MGRNVFSHDY721VFWCGDFNYR IDLTYEEVFY FVKRQDWKKL LEFDQLQLQK SSGKIFKDFH EGAINFGPTY781KYDVGSAAYD TSDKCRTPAW TDRVLWWRKK HPFDKTAGEL NLLDSDLDVD TKVRHTWSPG841ALQYYGRAEL QASDHRPVLA IVEVEVQEVD VGARERVFQE VSSFQGPLDA TVVVNLQSPT901LEEKNEFPED LRTELMQTLG SYGTIVLVRI NQGQMLVTFA DSHSALSVLD VDGMKVKGRA961VKIRPKTKDW LKGLREEIIR KRDSMAPVSP TANSCLLEEN FDFTSLDYES EGDILEDDED1021YLVDEFNQPG VSDSELGGDD LSDVPGPTAL APPSKSPALT KKKQHPTYKD DADLVELKRE1081LEAVGEFRHR SPSRSLSVPN RPRPPQPPQR PPPPTGLMVK KSASDASISS GTHGQYSILQ1141TARLLPGAPQ QPPKARTGIS KPYNVKQIKT TNAQEAEAAI RCLLEARGGA SEEALSAVAP1201RDLEASSEPE PTPGAAKPET PQAPPLLPRR PPPRVPAIKK PTLRRTGKPL SPEEQFEQQT1261VHFTIGPPET SVEAPPVVTA PRVPPVPKPR TFQPGKAAER PSHRKPASDE APPGAGASVP1321PPLEAPPLVP KVPPRRKKSA PAAFHLQVLQ SNSQLLQGLT YNSSDSPSGH PPAAGTVFPQ1381GDFLSTSSAT SPDSDGTKAM KPEAAPLLGD YQDPFWNLLH HPKLLNNTWL SKSSDPLDSG1441TRSPKRDPID PVSAGASAAK AELPPDHGHK TLGHWVTISD QEKRTALQVF DPLAKTSGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
1496 aa + 34 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.