Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SYNE2 / Nesprin-2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G104089-100
Catalog Number: LS-G104089
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Nesprin 2(SYNE2) (NM_182910), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- DKFZP434H2235 | KIAA1011 | Nesprin 2 | NUA | NUANCE | Polytrophin | SYNE2 | Nesprin-2 | TROPH | Protein NUANCE | EDMD5 | Nesp2 | SYNE-2
- Origin Species
- Human
- NCBI GenBank
- AL080133
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 48 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MEGDRNVPPV PPASSTPYKP PYGKLLLPPG TDGGKEGPRV LNGNPQQEDG GLAGITEQQS61GAFDRWEMIQ AQELHNKLKI KQNLQQLNSD ISAITTWLKK TEAELEMLKM AKPPSDIQEI121ELRVKRLQEI LKAFDTYKAL VVSVNVSSKE FLQTESPEST ELQSRLRQLS LLWEAAQGAV181DSWRGGLRQS LMQCQGSKTR PRSDVLFFKD FHQLSQNLLL WLASAKNRRQ KAHVTDPKAD241PRALLECRRE LMQLEKELVE RQPQVDMLQE ISNSLLIKGH GEDCIEAEEK VHVIEKKLKQ301LREQVSQDLM ALQGTQNPAS PLPSFDEVDS GDQPPATSVP APRAKFRAVR TTEGEEETES361RVPGSTRPQR SFLSRVVRAA LPLQLLLLLL LLLACLLPSS EEDYSCTQAN NFARSFYPML421RYTNGPPPTTRTRPLEQKLISEEDLAANDILDYKDDDDKV
429 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.