Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SYNCAM / CADM1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G119243-100
Catalog Number: LS-G119243
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SynCAM(CADM1) (NM_001098517), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- BL2 | Cell adhesion molecule 1 | CADM1 | IGSF4 | Necl-2 | IGSF4A | RA175 | SYNCAM | SynCAM1 | STSLC-1 | TSLC1/Nectin-like 2/IGSF4 | NECL2 | Nectin-like protein 2 | TSLC-1 | TSLC1 | Nectin-like 2 | SgIGSF | ST17 | Truncated CADM1 protein
- Origin Species
- Human
- NCBI GenBank
- NM_014333
- SwissProt
- Q9BY67
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 45.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MASVVLPSGS QCAAAAAAAA PPGLRLRLLL LLFSAAALIP TGDGQNLFTK DVTVIEGEVA61TISCQVNKSD DSVIQLLNPN RQTIYFRDFR PLKDSRFQLL NFSSSELKVS LTNVSISDEG121RYFCQLYTDP PQESYTTITV LVPPRNLMID IQKDTAVEGE EIEVNCTAMA SKPATTIRWF181KGNTELKGKS EVEEWSDMYT VTSQLMLKVH KEDDGVPVIC QVEHPAVTGN LQTQRYLEVQ241YKPQVHIQMT YPLQGLTREG DALELTCEAI GKPQPVMVTW VRVDDEMPQH AVLSGPNLFI301NNLNKTDNGT YRCEASNIVG KAHSDYMLYV YDSRAGEEGS IRAVDHAVIG GVVAVVVFAM361LCLLIILGRY FARHKGTYFT HEAKGADDAA DADTAIINAE GGQNNSEEKK EYFITRTRPLEQKLISEEDLAANDILDYKDDDDKV
414 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.