Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SYN3 / Synapsin III Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G107480-100
Catalog Number: LS-G107480
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Synapsin III(SYN3) (NM_133633), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CN28H9.2 (synapsin III) | SYN3 | Synapsin III | Synapsin-3
- Origin Species
- Human
- NCBI GenBank
- NM_003490
- SwissProt
- O14994
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 48.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MNFLRRRLSD SSFMANLPNG YMTDLQRPDS STSSPASPAM ERRHPQPLAA SFSSPGSSLF61SSLSSAMKQA PQATSGLMEP PGPSTPIVQR PRILLVIDDA HTDWSKYFHG KKVNGEIEIR121VEQAEFSELN LAAYVTGGCM VDMQVVRNGT KVVSRSFKPD FILVRQHAYS MALGEDYRSL181VIGLQYGGLP AVNSLYSVYN FCSKPWVFSQ LIKIFHSLGP EKFPLVEQTF FPNHKPMVTA241PHFPVVVKLG HAHAGMGKIK VENQLDFQDI TSVVAMAKTY ATTEAFIDSK YDIRIQKIGS301NYKAYMRTSI SGNWKANTGS AMLEQVAMTE RYRLWVDSCS EMFGGLDICA VKAVHSKDGR361DYIIEVMDSS MPLIGEHVEE DRQLMADLVV SKMSQLPMPG GTAPSPLRPW APQIKSAKSP421GQAQLGPQLG QPQPRPPPQA NLSPTRTRPL EQKLISEEDL AANDILDYKD DDDKV
475 aa
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.