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SYBU Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SYBU Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G119154-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Syntabulin(SYBU) (NM_001099756), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
GOLSYN | GOLSYN A protein | GOLSYN C protein | GOLSYN B protein | SNPHL | SYBU | Syntabulin | Microtubule-associated protein | OCSYN | FLJ20366 | Golgi-localized protein | Syntaxin-1-binding protein
Origin Species
Human
NCBI GenBank
NM_017786
SwissProt
Q9NX95

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
71.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSNFEKEHRV QHHDKEISRS RIPRLILRPH MPQQQHKVSP ASESPFSEEE SREFNPSSSG
61RSARTVSSNS FCSDDTGCPS SQSVSPVKTP SDAGNSPIGF CPGSDEGFTR KKCTIGMVGE
121GSIQSSRYKK ESKSGLVKPG SEADFSSSSS TGSISAPEVH MSTAGSKRSS SSRNRGPHGR
181SNGASSHKPG SSPSSPREKD LLSMLCRNQL SPVNIHPSYA PSSPSSSNSG SYKGSDCSPI
241MRRSGRYMSC GENHGVRPPN PEQYLTPLQQ KEVTVRHLKT KLKESERRLH ERESEIVELK
301SQLARMREDW IEEECHRVEA QLALKEARKE IKQLKQVIET MRSSLADKDK GIQKYFVDIN
361IQNKKLESLL QSMEMAHSGS LRDELCLDFP CDSPEKSLTL NPPLDTMADG LSLEEQVTGE
421GADRELLVGD SIANSTDLFD EIVTATTTES GDLELVHSTP GANVLELLPI VMGQEEGSVV
481VERAVQTDVV PYSPAISELI QSVLQKLQDP CPSSLASPDE SEPDSMESFP ESLSALVVDL
541TPRNPNSAIL LSPVETPYAN VDAEVHANRL MRELDFAACV EERLDGVIPL ARGGVVRQYW
601SSSFLVDLLA VAAPVVPTVL WAFSTQRGGT DPVYNIGALL RGCCVVALHS LRRTAFRIKT
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

660 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.