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SWAP70 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SWAP70 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G113492-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SWAP70 (NM_015055), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA0640 | HSPC321 | SWAP-70 | Switch-associated protein 70 | SWAP70
Origin Species
Human
NCBI GenBank
NM_015055
SwissProt
Q9UH65

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
68.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGSLKEELLK AIWHAFTALD QDHSGKVSKS QLKVLSHNLC TVLKVPHDPV ALEEHFRDDD
61EGPVSNQGYM PYLNRFILEK VQDNFDKIEF NRMCWTLCVK KNLTKNPLLI TEEDAFKIWV
121IFNFLSEDKY PLIIVSEEIE YLLKKLTEAM GGGWQQEQFE HYKINFDDSK NGLSAWELIE
181LIGNGQFSKG MDRQTVSMAI NEVFNELILD VLKQGYMMKK GHRRKNWTER WFVLKPNIIS
241YYVSEDLKDK KGDILLDENC CVESLPDKDG KKCLFLVKCF DKTFEISASD KKKKQEWIQA
301IHSTIHLLKL GSPPPHKEAR QRRKELRKKQ LAEQEELERQ MKELQAANES KQQELEAVRK
361KLEEAASRAA EEEKKRLQTQ VELQARFSTE LEREKLIRQQ MEEQVAQKSS ELEQYLQRVR
421ELEDMYLKLQ EALEDERQAR QDEETVRKLQ ARLLEEESSK RAELEKWHLE QQQAIQTTEA
481EKQELENQRV LKEQALQEAM EQLEQLELER KQALEQYEEV KKKLEMATNK TKSWKDKVAH
541HEGLIRLIEP GSKNPHLITN WGPAAFTEAE LEEREKNWKE KKTTETRTRP LEQKLISEED
601LAANDILDYK DDDDKV

616 aa

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.