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SVOP Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SVOP Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all SVOP antibodies →
$504.00each
Catalog Number: LS-G101593-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SVOP (NM_018711), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
SV2-related protein | SVOP
Origin Species
Human
NCBI GenBank
BC033587
SwissProt
Q8N4V2

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
60.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEEDLFQLRQ LPVVKFRRTG ESARSEDDTA SGEHEVQIEG VHVGLEAVEL DDGAAVPKEF
61ANPTDDTFMV EDAVEAIGFG KFQWKLSVLT GLAWMADAME MMILSILAPQ LHCEWRLPSW
121QVALLTSVVF VGMMSSSTLW GNISDQYGRK TGLKISVLWT LYYGILSAFA PVYSWILVLR
181GLVGFGIGGV PQSVTLYAEF LPMKARAKCI LLIEVFWAIG TVFEVVLAVF VMPSLGWRWL
241LILSAVPLLL FAVLCFWLPE SARYDVLSGN QEKAIATLKR IATENGAPMP LGKLIISRQE
301DRGKMRDLFT PHFRWTTLLL WFIWFSNAFS YYGLVLLTTE LFQAGDVCGI SSRKKAVEAK
361CSLACEYLSE EDYMDLLWTT LSEFPGVLVT LWIIDRLGRK KTMALCFVIF SFCSLLLFIC
421VGRNVLTLLL FIARAFISGG FQAAYVYTPE VYPTATRALG LGTCSGMARV GALITPFIAQ
481VMLESSVYLT LAVYSGCCLL AALASCFLPI ETKGRGLQES SHREWGQEMV GRGMHGAGVT
541RSNSGSQE
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

548 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.