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SV2C Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SV2C Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all SV2C antibodies →
$725.00each
Catalog Number: LS-G113543-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SV2C (NM_014979), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA1054 | Synaptic vesicle protein 2C | SV2C
Origin Species
Human
NCBI GenBank
NM_014979
SwissProt
Q496J9

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
82.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEDSYKDRTS LMKGAKDIAR EVKKQTVKKV NQAVDRAQDE YTQRSYSRFQ DEEDDDDYYP
61AGETYNGEAN DDEGSSEATE GHDEDDEIYE GEYQGIPSMN QAKDSIVSVG QPKGDEYKDR
121RELESERRAD EEELAQQYEL IIQECGHGRF QWALFFVLGM ALMADGVEVF VVGFVLPSAE
181TDLCIPNSGS GWLGSIVYLG MMVGAFFWGG LADKVGRKQS LLICMSVNGF FAFLSSFVQG
241YGFFLFCRLL SGFGIGGAIP TVFSYFAEVL AREKRGEHLS WLCMFWMIGG IYASAMAWAI
301IPHYGWSFSM GSAYQFHSWR VFVIVCALPC VSSVVALTFM PESPRSLLEV GKHDEAWMIL
361KLIHDTNMRA RGQPEKVFTV NKIKTPKQID ELIEIESDTG TWYRRCFVRI RTELYGIWLT
421FMRCFNYPVR DNTIKLTIVW FTLSFGYYGL SVWFPDVIKP LQSDEYALLT RNVERDKYAN
481FTINFTMENQ IHTGMEYDNG RFIGVKFKSV TFKDSVFKSC TFEDVTSVNT YFKNCTFIDT
541VFDNTDFEPY KFIDSEFKNC SFFHNKTGCQ ITFDDDYSAY WIYFVNFLGT LAVLPGNIVS
601ALLMDRIGRL TMLGGSMVLS GISCFFLWFG TSESMMIGML CLYNGLTISA WNSLDVVTVE
661LYPTDRRATG FGFLNALCKA AAVLGNLIFG SLVSITKSIP ILLASTVLVC GGLVGLCLPD
721TRTQVLM
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

727 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.