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SV2B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SV2B Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G101264-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SV2B (NM_014848), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
HsT19680 | KIAA0735 | SV2B | Synaptic vesicle protein 2B
Origin Species
Human
NCBI GenBank
NM_014848
SwissProt
Q7L1I2

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
77.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDDYKYQDNY GGYAPSDGYY RGNESNPEED AQSDVTEGHD EEDEIYEGEY QGIPHPDDVK
61AKQAKMAPSR MDSLRGQTDL MAERLEDEEQ LAHQYETIMD ECGHGRFQWI LFFVLGLALM
121ADGVEVFVVS FALPSAEKDM CLSSSKKGML GMIVYLGMMA GAFILGGLAD KLGRKRVLSM
181SLAVNASFAS LSSFVQGYGA FLFCRLISGI GIGGALPIVF AYFSEFLSRE KRGEHLSWLG
241IFWMTGGLYA SAMAWSIIPH YGWGFSMGTN YHFHSWRVFV IVCALPCTVS MVALKFMPES
301PRFLLEMGKH DEAWMILKQV HDTNMRAKGT PEKVFTVSNI KTPKQMDEFI EIQSSTGTWY
361QRWLVRFKTI FKQVWDNALY CVMGPYRMNT LILAVVWFAM AFSYYGLTVW FPDMIRYFQD
421EEYKSKMKVF FGEHVYGATI NFTMENQIHQ HGKLVNDKFT RMYFKHVLFE DTFFDECYFE
481DVTSTDTYFK NCTIESTIFY NTDLYEHKFI NCRFINSTFL EQKEGCHMDL EQDNDFLIYL
541VSFLGSLSVL PGNIISALLM DRIGRLKMIG GSMLISAVCC FFLFFGNSES AMIGWQCLFC
601GTSIAAWNAL DVITVELYPT NQRATAFGIL NGLCKFGAIL GNTIFASFVG ITKVVPILLA
661AASLVGGGLI ALRLPETREQ VLM
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

683 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.