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SUSD5 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SUSD5 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G113138-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SUSD5 (NM_015551), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA0527 | Sushi domain containing 5 | SUSD5
Origin Species
Human
NCBI GenBank
AB011099
SwissProt
O60279

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
67.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MTAEGPSPPA RWHRRLPGLW AAALLLLGLP RLSVRADGKF FVLESQNGSQ GLQLEAARLS
61CKSRGAHLAS ADELRRVVQD CSFAVCTTGW LADGTLGTTV CSKGSGEQQI MRAVDVRIES
121NPVPGGTYSA LCIKDEEKPC GDPPSFPHTI LQGRTGLEMG DELLYVCAPG HIMGHRETAF
181TLLCNSCGEW YGLVQACGKD EAEAHIDYED NFPDDRSVSF RELMEDSRTE ADEDRGQGDS
241SEEAPKQDRL VSISVGRENI ARDKVFVPTT GLPGAGSSVP ADSPGSRLLQ KHLFWFPAEA
301FHKPGLEKEV DDDTKKQFSA GDNHSGVKLV PGEPETKVIY GNTDGPSGPF VGKNDSKAGD
361PVVSSSDESW LDGYPVTEGA WRKTEAEEEE DGDRGDGSVG LDENVLVTPD QPILVEVKKP
421KSSTLTPSEG MTHSSVLPSQ MLDVEALALR PVNASETEGI GDGDLTKYQS TLPWRFITEE
481SPMATLSYEL TSSTLEILTV NTVKQTPNHI PSTIMATTQP PVETTVPEIQ DSFPYLLSED
541FFGQEGPGPG ASEELHPTLE SCVGDGCPGL SRGPVIATIV TVLCLLLLLA GVGMVWGYRK
601CQHKSSVYKL NVGQRQARHY HQQIEMEKV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

629 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.