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SUPV3L1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SUPV3L1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G117515-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SUPV3L1 (NM_003171), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
SUPV3L1 | SUV3 | SUV3-like protein 1
Origin Species
Human
NCBI GenBank
NM_003171
SwissProt
Q8IYB8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
87.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSFSRALLWA RLPAGRQAGH RAAICSALRP HFGPFPGVLG QVSVLATASS SASGGSKIPN
61TSLFVPLTVK PQGPSADGDV GAELTRPLDK NEVKKVLDKF YKRKEIQKLG ADYGLDARLF
121HQAFISFRNY IMQSHSLDVD IHIVLNDICF GAAHADDLFP FFLRHAKQIF PVLDCKDDLR
181KISDLRIPPN WYPDARAMQR KIIFHSGPTN SGKTYHAIQK YFSAKSGVYC GPLKLLAHEI
241FEKSNAAGVP CDLVTGEERV TVQPNGKQAS HVSCTVEMCS VTTPYEVAVI DEIQMIRDPA
301RGWAWTRALL GLCAEEVHLC GEPAAIDLVM ELMYTTGEEV EVRDYKRLTP ISVLDHALES
361LDNLRPGDCI VCFSKNDIYS VSRQIEIRGL ESAVIYGSLP PGTKLAQAKK FNDPNDPCKI
421LVATDAIGMG LNLSIRRIIF YSLIKPSINE KGERELEPIT TSQALQIAGR AGRFSSRFKE
481GEVTTMNHED LSLLKEILKR PVDPIRAAGL HPTAEQIEMF AYHLPDATLS NLIDIFVDFS
541QVDGQYFVCN MDDFKFSAEL IQHIPLSLRV RYVFCTAPIN KKQPFVCSSL LQFARQYSRN
601EPLTFAWLRR YIKWPLLPPK NIKDLMDLEA VHDVLDLYLW LSYRFMDMFP DASLIRDLQK
661ELDGIIQDGV HNITKLIKMS ETHKLLNLEG FPSGSQSRLS GTLKSQARRT RGTKALGSKA
721TEPPSPDAGE LSLASRLVQQ GLLTPDMLKQ LEKEWMTQQT EHNKEKTESG THPKGTRRKK
781KEPDSD
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

786 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.